6. John Colby, Howard Dalton. // Ann. Rev. Microbiol. - 1979. - V.33. - P.481-517.
7. Webster. I.// Biotechnol. and Bioengeneery.- 1983. - V.25. - C.31-50.
8. Мосин О.В., Карнаухова Е.Н., Пшеничникова А.Б.,Складнев Д.А., Акимова О.Л. // Биотехнология. - 1993. - N.9. - С.16-20.
9. Егорова Т.А., Мосин О.В., Еремин С.В., Карнаухова Е.Н.,Звонкова Е.Н., Швец В.И. // Биотехнология. - 1993. -N.8. - С.21-25.
10. Karnaukhova E.N., Mosin O.V., Reshetova O.S.Biosynthetic production of stable isotope labelled amino acids, using methylotroph Methylobacillus flagellatum. // Amino Acids. - 1993. - V.5. - No.1. - P.125.
11. Миллер Дж. // Эксперименты в молекулярной генетике.- М.:Мир, -1976. - С.393.
12. Karnaukhova E.N., Reshetova O.S., Semenov S.Y., Skladnev D.A., Tsygankov Y.D. // Amino Acids. - 1994. - V.6. - P.165-176.
13. Bligh E.G., Dyer W.J. // Can. J. Biochem. Physiol. - 1959. - V.37. - N.8. - P.911-918.
14. Грачева И.М., Гаврилова Н.Н., Иванова Л.А. //Технология микробных белковых препаратов, аминокислот и жиров. - М.:Пищевая пром-ть, -1980. - С.291-295.
15. Зорина А.В, Бабусенко Е.С. Химический состав биомассы метилотрофных бактерий, современные проблемы биотехнологии микроорганизмов. // Тезисы докл. молодых ученых. - Рига.,1987. -С.35-40.
О. V. MOSIN
Мoscow State Academy of Fine Chemical Technology named after М.V. Lomonosov, 117571.
THE GROWTH OF BACTERIUM OF Basillus subtilis AND BIOSYNTHESIS OF INOSINE ON HIGHLY DEUTERATED MEDIUM.
The possibility for biosynthetic preparation of deuterated inosine obtained from the culture medium of inosine-producing strain B. subtilis was described. The method is based on using the deutero-compounds of facultative methylotrophic bacterium Brevibacterium methylicum obtained from the completely deuterated medium containing 98 v/v% 2Н2O и 2 об. v/v% С2Н3О2Н as a source of growth factors by cultivation of inosine-producing strain. The data on growth of strain of B. subtilis and biosynthesis of inosine on highly deuterated medium containing maximum concentratioms of 2H2O and deutero-compounds extracted from methylotrophic bacteria were described. The evaluation of deuterium enrichment of inosine was carried out using mass-spectrometry FAB. The data obtained testified to a high efficiency of deuterium incorporation into inosine (62,5 % hydrogen atoms are substituted with deuterium in molecule of inosine)