light and heavy peaks of [M]+of methyl ester of N-DNS phenylalanine is five units. This is in agreement with the earlier obtained result and the data on the level of deutera-tion of initial [2,3,4,5,6-2H5]phenylalanine added into the growth medium.
Thus, these data indicate a high efficiency of incorporation of 2H-labeled aromatic amino acids into the BR molecule. Completely deuterated protein preparations for reconstruction (into 2H2O) of functionally active systems of membrane proteins with purified 2H-labeled lipids and other deuterated biologically active compounds are proposed to be obtained using the method elaborated. In the future, these studies will provide the means for solving the problem of functioning of 2H-Iabeled BR in the composition of artificially constructed membranes under conditions of deuterium-saturated medium.
This work was supported by grant no. 1B-22-866 ("High chemical technologies"). We are grateful to Dr. B.M. Polanuer (GNU GENETICA) for careful attention and helpful remarks in discussions of the results.
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