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Incorporation of 23456-2H5Phenylalanine 35-2H2Tyrosine and 24567-2H5Tryptophan into the Bacteriorhodopsin (стр. 3 из 3)


light and heavy peaks of [M]+of methyl ester of N-DNS phenylalanine is five units. This is in agreement with the earlier obtained result and the data on the level of deutera-tion of initial [2,3,4,5,6-2H5]phenylalanine added into the growth medium.

Thus, these data indicate a high efficiency of incor­poration of 2H-labeled aromatic amino acids into the BR molecule. Completely deuterated protein prepara­tions for reconstruction (into 2H2O) of functionally active systems of membrane proteins with purified 2H-labeled lipids and other deuterated biologically active compounds are proposed to be obtained using the method elaborated. In the future, these studies will pro­vide the means for solving the problem of functioning of 2H-Iabeled BR in the composition of artificially con­structed membranes under conditions of deuterium-sat­urated medium.

ACKNOWLEDGMENTS

This work was supported by grant no. 1B-22-866 ("High chemical technologies"). We are grateful to Dr. B.M. Polanuer (GNU GENETICA) for careful attention and helpful remarks in discussions of the results.

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